Journal: Life

Article Title: The Timing and Effects of Low-Dose Ethanol Treatment on Acetaminophen-Induced Liver Injury

doi: 10.3390/life11101094

Figure Lengend Snippet: The effects of timing of ethanol treatment on neutrophil ( A ) and macrophage ( B ) infiltration in acetaminophen-induced liver injury. Mice were administrated APAP (300 mg/kg) or an equal volume of saline (control) intraperitoneally and treated with 0.5 g/kg of ethanol before and after 1 h. Mice were sacrificed after 16 h of APAP challenge. The liver sections were immunostained with ( A ) Ly6G or ( B ) Mac-2 antibody (brown). Representative immunohistochemical staining of the liver tissues obtained from different groups. (200× magnifications are shown). Quantification of positive inflammatory cells was analyzed under high power field (HPF). Each value represents the mean ± SEM; n = 6 for each group. # p < 0.05, ### p < 0.005 vs. control group; * p < 0.05, *** p < 0.005 vs. APAP group.

Article Snippet: For immunohistochemical staining, the sections were incubated with primary antibody against Ly6G (neutrophil; 1:500; BD Biosciences Pharmingen, San Diego, CA, USA) or Mac-2 (macrophage; 1:500; eBioscience, Inc., San Diego, CA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: Life

Article Title: The Timing and Effects of Low-Dose Ethanol Treatment on Acetaminophen-Induced Liver Injury

doi: 10.3390/life11101094

Figure Lengend Snippet: The effects of different ethanol administration times on neutrophil ( A ) and macrophages ( B ) infiltration in acetaminophen-induced liver injury. Mice were administrated APAP (300 mg/kg) or an equal volume of saline (control) intraperitoneally and treated with 0.5 g/kg of ethanol after 1, 2, or 4 h. Mice were sacrificed after 16 h of APAP challenge. The liver sections were immunostained with ( A ) Ly6G or ( B ) Mac-2 antibody (brown). Representative immunohistochemical staining of the liver tissues obtained from different groups. (200× magnifications are shown). Quantification of positive inflammatory cells was analyzed under HPF. Each value represents the mean ± SEM; n = 6 for each group. # p < 0.05, ## p < 0.01, ### p < 0.005 vs. control group; *** p < 0.005 vs. APAP group.

Article Snippet: For immunohistochemical staining, the sections were incubated with primary antibody against Ly6G (neutrophil; 1:500; BD Biosciences Pharmingen, San Diego, CA, USA) or Mac-2 (macrophage; 1:500; eBioscience, Inc., San Diego, CA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: JACC: Basic to Translational Science

Article Title: Proteolysis of Von Willebrand Factor Influences Inflammatory Endothelial Activation and Vascular Compliance in Atherosclerosis

doi: 10.1016/j.jacbts.2020.08.009

Figure Lengend Snippet: Histology From the Aortic Root Histology at the level of the aortic sinuses at 30 weeks of age that include quantitative measurement of plaque area on Masson trichrome staining (A) , plaque macrophage area by Mac-2 staining (B) , plaque collagen area by Masson trichrome (C) , and platelet number by CD41 staining (D) seen per aortic root section. Values are mean ± SEM. ∗p < 0.05 versus WT and LDL-R −/− ; † p < 0.05 versus LDL-R −/− on WSD; ‡ p < 0.05 versus WT mice. All p values are post hoc (analysis of variance, p < 0.05) analysis with unpaired Student's t-tests. n = 6 for each animal group. (E) Examples illustrating group-related differences in histology from the aortic root showing plaque area by Masson trichrome (top row) , fluorescent immunohistochemistry for Mac-2 (bottom row) , and merged bright-field and Mac-2 (center row) . Bars = 150 μm. DAPI = 4′,6-diamidino-2-phenylindole; other abbreviations as in Figure 1 .

Article Snippet: Immunohistochemistry was performed with rat anti-mouse primary monoclonal antibody against Mac-2 (M3/38, Thermo Fisher Scientific, Waltham, Massachusetts) for macrophages and dendritic cells, against CD41 (ab33661, Abcam) for platelets, and against vWF (ab6994, Abcam).

Techniques: Staining, Immunohistochemistry

Journal: JACC: Basic to Translational Science

Article Title: Proteolysis of Von Willebrand Factor Influences Inflammatory Endothelial Activation and Vascular Compliance in Atherosclerosis

doi: 10.1016/j.jacbts.2020.08.009

Figure Lengend Snippet: Correlation Between Arterial Phenotype on Molecular Imaging and Histology Correlations between contrast-enhanced ultrasound molecular imaging signal for platelet GPIbα and either plaque area (A) or plaque Mac-2 (B) on histology from the aortic sinuses at 30 weeks of age. Data are from all histological samples; circles represent WT mice; open squares represent LDL-R −/− mice; triangles represent LDL-R −/− mice on WSD; and solid squares represent LDL-R −/− AD13 −/− mice on WSD. Abbreviations as in Figures 1 and .

Article Snippet: Immunohistochemistry was performed with rat anti-mouse primary monoclonal antibody against Mac-2 (M3/38, Thermo Fisher Scientific, Waltham, Massachusetts) for macrophages and dendritic cells, against CD41 (ab33661, Abcam) for platelets, and against vWF (ab6994, Abcam).

Techniques: Imaging

Journal: JACC: Basic to Translational Science

Article Title: Proteolysis of Von Willebrand Factor Influences Inflammatory Endothelial Activation and Vascular Compliance in Atherosclerosis

doi: 10.1016/j.jacbts.2020.08.009

Figure Lengend Snippet: Histologic Changes Produced by AD13 Treatment Histology from the aortic root at the level of the aortic sinuses in LDL-R −/− on WSD at 30 weeks of age comparing untreated and AD13-treated (2 weeks by osmotic minipump), include quantitative measurement of plaque area on Masson trichrome staining (A) , plaque macrophage area by Mac-2 staining (B) , plaque collagen area by Masson trichrome (C) , and platelet number by CD41 staining (D) seen per aortic root section. Values are mean ± SEM. ∗p < 0.05 versus WT and LDL-R −/− by unpaired Student's t-tests. n = 6 for each animal group. (E) Examples illustrating Masson trichrome (left) , fluorescent immunohistochemistry for Mac-2 (center) , and CD41 for platelets (right) are shown for comparison to Figure 4 . Bars = 150 μm. Abbreviations as in Figures 1 , , and .

Article Snippet: Immunohistochemistry was performed with rat anti-mouse primary monoclonal antibody against Mac-2 (M3/38, Thermo Fisher Scientific, Waltham, Massachusetts) for macrophages and dendritic cells, against CD41 (ab33661, Abcam) for platelets, and against vWF (ab6994, Abcam).

Techniques: Produced, Staining, Immunohistochemistry